Advanced Lab Technologies Core

Advanced Lab Technologies Core

The mission of the ALT Core is to provide HU CFAR members with access and training to state-of-the-art technologies for HIV research.

Services

Specimen collection
  • Cytometry (50 color analyses and color sorting) 
  • Microscopy (multispectral and live tissue imaging, confocal microscopy)
  • Genomics (RNAseq, ChIPseq, single cell resolution, CRISPR screening) 
  • BSL3 facilities for HIV/TB co-infection and SARS-CoV-2 studies (including cytometry/sorting, live-cell microscopy, 10x genomics, and Luminex)
  • Access to training in state-of-the art technologies, including computational assistance and BSL3 consultation, training, and technical execution of experiments
  • HIV-1 Neutralizing Antibody Core, a resource for assessing the breadth and potency of HIV-1 neutralizing antibodies, and for characterizing the neutralization phenotype of HIV-1 isolates
  • Spatial profiling (10x Visium)
Two people discussing
  • Training to support the immediate use of new equipment
  • Quarterly symposia and training sessions around technological advances to foster synergy among CFAR members
Keyboard
  • Cloud computing transfer and sharing of datasets
  • Synergistic sharing of raw data across projects
  • Standardization of protocols and file structures
  • Creation of a common platform for simple web access and sharing

Core Site Services

BSL3 at Ragon Institute of Mass General, MIT, and Harvard
  • Training and daily access fees
  • Mouse daily housing fees
  • Technical execution of experiments
  • BSL3 live flow (analytical or sorting)
  • BSL3 live microscopy

To request access to these core site services contact Amy Barczak.

Flow and Microscopy Core at Ragon Institute of Mass General, MIT, and Harvard/Beth Israel Deaconess Medical Center
  • Training fees
  • Hourly microscopy fees
  • Assisted microscopy
  • Hourly flow cytometry fees (analytical, high-throughput)
  • Assisted flow sorting

To request access to these core site services contact Daniel Lingwood for Flow/Microscopy at Ragon and Sizun Jiang for Flow at BIDMC.

Genomics Core at Dana-Farber Cancer Institute
  • NGS sample library preparation fees
  • NGS sequencing fees (all applications including bulk and single-cell RNAseq)
  • Bioinformatics fees

To request access to these core site services contact Zach Herbert.

Neutralizing Antibody Core at Beth Israel Deaconess Medical Center
  • Assisted virus panel testing plan
  • Assay fees (technical execution of experiments)

To request access to these core site services contact Mike Seaman.

Contact us to use our services

5 results found.

Amy K. Barczak, MD

Associate Director, HU CFAR Advanced Lab Technologies CoreHU CFAR Executive Committee MemberAssistant Professor of Medicine, Harvard Medical Schoolabarczak@partners.org

Zachary T. Herbert, MS

Associate Director, HU CFAR Advanced Lab Technologies CoreHU CFAR Executive Committee MemberResearch Associate in Medicine, Dana-Farber Cancer Centerzherbert@mail.dfci.harvard.edu

Sizun Jiang, PhD

Associate Director, HU CFAR Advanced Lab Technologies CoreAssistant Professor of Medicine, Beth Israel Deaconess Medical Centersjiang3@bidmc.harvard.edu

Daniel Lingwood, PhD

Director, HU CFAR Advanced Lab Technologies CoreHU CFAR Executive Committee MemberAssociate Professor of Medicine, Harvard Medical Schooldlingwood@mgh.harvard.edu

Michael S. Seaman PhD

Associate Director, HU CFAR Advanced Lab Technologies CoreAssociate Professor of Medicine, Beth Israel Deaconess Medical Centermseaman@bidmc.harvard.edu
Pointing pen towards a slide

Contact us with questions:

Email: cfar@harvard.edu

Harvard University Center for AIDS Research
42 Church Street
Cambridge, MA 02138 

Phone: 617-384-9039
Fax: 617-496-1833