Advanced Lab Technologies Core
The mission of the ALT Core is to provide HU CFAR members with access and training to state-of-the-art technologies for HIV research.
Services
Access to state-of-the art technologies and resources
- Cytometry (50 color analyses and color sorting)
- Microscopy (multispectral and live tissue imaging, confocal microscopy)
- Genomics (RNAseq, ChIPseq, single cell resolution, CRISPR screening)
- BSL3 facilities for HIV/TB co-infection and SARS-CoV-2 studies (including cytometry/sorting, live-cell microscopy, 10x genomics, and Luminex)
- Access to training in state-of-the art technologies, including computational assistance and BSL3 consultation, training, and technical execution of experiments
- HIV-1 Neutralizing Antibody Core, a resource for assessing the breadth and potency of HIV-1 neutralizing antibodies, and for characterizing the neutralization phenotype of HIV-1 isolates
- Spatial profiling (10x Visium)
User Training for Investigators
- Training to support the immediate use of new equipment
- Quarterly symposia and training sessions around technological advances to foster synergy among CFAR members
Data Sharing and Distribution Tools
- Cloud computing transfer and sharing of datasets
- Synergistic sharing of raw data across projects
- Standardization of protocols and file structures
- Creation of a common platform for simple web access and sharing
Core Site Services
BSL3 at Ragon Institute of Mass General, MIT, and Harvard
- Training and daily access fees
- Mouse daily housing fees
- Technical execution of experiments
- BSL3 live flow (analytical or sorting)
- BSL3 live microscopy
To request access to these core site services contact Amy Barczak.
Flow and Microscopy Core at Ragon Institute of Mass General, MIT, and Harvard/Beth Israel Deaconess Medical Center
- Training fees
- Hourly microscopy fees
- Assisted microscopy
- Hourly flow cytometry fees (analytical, high-throughput)
- Assisted flow sorting
To request access to these core site services contact Daniel Lingwood for Flow/Microscopy at Ragon and Sizun Jiang for Flow at BIDMC.
Genomics Core at Dana-Farber Cancer Institute
- NGS sample library preparation fees
- NGS sequencing fees (all applications including bulk and single-cell RNAseq)
- Bioinformatics fees
To request access to these core site services contact Zach Herbert.
Neutralizing Antibody Core at Beth Israel Deaconess Medical Center
- Assisted virus panel testing plan
- Assay fees (technical execution of experiments)
To request access to these core site services contact Mike Seaman.
Contact us to use our services
Amy K. Barczak, MD
Associate Director, HU CFAR Advanced Lab Technologies CoreHU CFAR Executive Committee MemberAssistant Professor of Medicine, Harvard Medical Schoolabarczak@partners.org
Zachary T. Herbert, MS
Associate Director, HU CFAR Advanced Lab Technologies CoreHU CFAR Executive Committee MemberResearch Associate in Medicine, Dana-Farber Cancer Centerzherbert@mail.dfci.harvard.edu
Sizun Jiang, PhD
Associate Director, HU CFAR Advanced Lab Technologies CoreAssistant Professor of Medicine, Beth Israel Deaconess Medical Centersjiang3@bidmc.harvard.edu
Daniel Lingwood, PhD
Director, HU CFAR Advanced Lab Technologies CoreHU CFAR Executive Committee MemberAssociate Professor of Medicine, Harvard Medical Schooldlingwood@mgh.harvard.edu
Michael S. Seaman PhD
Associate Director, HU CFAR Advanced Lab Technologies CoreAssociate Professor of Medicine, Beth Israel Deaconess Medical Centermseaman@bidmc.harvard.edu
Contact us with questions:
Email: cfar@harvard.edu
Harvard University Center for AIDS Research
42 Church Street
Cambridge, MA 02138
Phone: 617-384-9039
Fax: 617-496-1833