Don't miss a chance to meet and greet Timothy Ray Brown "The Berlin Patient" and hear why he advocates for the HIV Cure.  Registration is required for this event.  Please register at https://fs3.formsite.com/divofaids/form274/index.html

 

 

 

 

Workshop on the Analysis of Biological Data for Studies on the Microbiome and Host-Gene Expression
Registration Deadline: 01 September 2018

Submit an application letter of interest and letter of support from a supervisor here: https://fs3.formsite.com/divofaids/form273/index.html
Download the workshop flyer

After your letters are received, you will be sent a link to the online registration.  There is a $100 USD registration fee.

This intensive, hands-on course will feature morning and afternoon sessions taught by expert faculty and technical assistants. All participants will acquire fundamental computational skills, appropriate study design, data interpretation, and overall literacy in bioinformatics.

Topics covered include: 

• Intro to computing
• Intro to R
• Computational study design
• Microbiome data analysis
• Transcriptional data analysis
• Differential Gene Expression

When: 08-12 October 2018, Morning Sessions: 9:00-12:00, Afternoon Sessions: 13:00-16:00

Where: AHRI Seminar Rooms 1&2,   Nelson R Mandela School of Medicine,   719 Umbilo Road, Durban, South Africa

Fee: There is $100 USD registration fee for this 5- day workshop. Breakfast, lunch and access to Amazon Web Services cloud infrastructure are included.

Requirements: Students are required to bring their own laptops to participate in the course. All software and data will be provided and managed by the course organizers. No previous experience in bioinformatics is needed.

 

Modern biological research projects regularly employee techniques capable of generating extremely large data sets. Specifically, microbiome investigations utilize amplicon surveys (16S rRNA, ITS, or 18S rRNA gene sequence) or metagenomic approaches to assess microbial ecology and gene expression studies take advantage of RNA-seq technology to identify differential gene regulation. Analysis of data resulting from any of these techniques requires proficiency in computational (UNIX, R) and statistical (exploratory data analysis, hypothesis testing, uni- and multivariant analysis) techniques.

The approach for analysis of both microbiome and gene expression analysis begins with appropriate understanding of the study design and metadata, proceeds through a process of data quality control and filtering, quantifies this filtered data and ultimately results in the production of tabular count data. In the case of microbiome studies, this is a table of each microbial taxa per sample and for gene expression studies a table of transcript counts per sample. Additional data types (e.g. taxonomic assignments, gene functional information) may also be created during this process and ultimately associated with or merged with the count table. These data types can then be explored using various plots and interrogated using statistical techniques. This Workshop provides instruction for how to proceed through each of these stages providing a strong foundation for working with count data and subsequent statistical analysis, plotting and interpretation.

Collaborating Institutions:

Harvard University Center for AIDS Research (CFAR)
Ragon Institute
Sub-Saharan African Network for TB/HIV Research Excellence (SANTHE)
Centre for the AIDS Program of Research in South Africa (CAPRISA)

Organizing Team:

Scott Handley, Washington University
Doug Kwon, Ragon Institute
Matt Hayward, Harvard University
Barry L. Hykes, Washington University
Chandni Desai, Washington University

SCHEDULE
Visit the evomics website for the latest schedule updates as the workshop approaches

DATE	DAY	TIME	PRESENTER	TOPIC	LOCATION
7 Oct	Sunday	6p – 10p	Everyone	Reception	AHRI Seminar Rooms 1&2
8 Oct	Monday	9a – 12p	Sophie Shaw	Introduction to UNIX	AHRI Seminar Rooms 1&2
	Monday	2p – 5p	Sophie Shaw	Introduction to Sequence Data and Sequence Data Quality Control	AHRI Seminar Rooms 1&2
9 Oct	Tuesday	9a – 12p	Lindsay Droit	Building Successful Sequencing Libraries	AHRI Seminar Rooms 1&2
	Tuesday	2p – 5p	Scott Handley	Introduction to Data Science with R	AHRI Seminar Rooms 1&2
10 Oct	Wednesday	9a – 12p	Scott Handley	Preprocessing Microbiome Data for Quantitative Microbiome Analysis (lecture)	AHRI Seminar Rooms 1&2
	Wednesday	2p – 5p	Scott Handley	Preprocessing Microbiome Data for Quantitative Microbiome Analysis (lab)	AHRI Seminar Rooms 1&2
11 Oct	Thursday	9a – 12p	Chandni Desai	Host Differential Gene Expression Analysis (lecture)	AHRI Seminar Rooms 1&2
	Thursday	2p – 5p	Chandni Desai & Barry Hykes	Host Differential Gene Expression Analysis (lab)	AHRI Seminar Rooms 1&2
12 Oct	Friday	9a – 12p	Matt Hayward	Metagenomic Analysis	AHRI Seminar Rooms 1&2
	Friday	2p – 5p	Everyone	Open Lab	AHRI Seminar Rooms 1&2

 

The understanding of the human microbiome continues to grow rapidly; however information on the role of HIV infection on changes in the microbiome, and the role of the microbiome in HIV transmission, prevention, pathogenesis and treatment is still limited.  While research in this area may be presented at major conferences, there is often limited time for in-depth discussion and debate among cross-disciplinary experts on new data and their implications. The aim of this workshop is to provide increased opportunities for discussion and exchange of knowledge following formal presentation of the latest research.

This 2 day event will have invited lectures by key research leaders from both the broader Microbiome field, as well as HIV/Microbiome investigators, oral abstract presentations and poster presentations.  The conference will be unique in its approach, as it will engage basic scientists and clinicians from the HIV field and leading scientists involved in microbiome researchs.  Registration is free.  There will also be a Young Investigator Scholarship program to support travel, to encourage participation of emerging investigators. 

The program including the speakers can be found at link to the website is below. 

http://www.virology-education.com/event/upcoming/4th-hiv-microbiome-workshop-2018/
For specific questions, please contact the Organizing Secretariat Kun-Chieh Wu <kun-chieh.wu@vironet.com